Coding

Part:BBa_I757010:Design

Designed by: iGEM team Freiburg 2007 and Jochen Hecky   Group: iGEM07_Freiburg   (2007-10-24)


b-lactamase TEM optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 722


Design Notes

between BioBrick 1.0 sites part is flanked 5' by NgoMIV and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions


Source

synthetic DNA by GeneArt

References